Quartz Glass Slides Basic Information
Quartz Glass Slides Material:
Slide is used in the experiment to place the experimental material of the glass, rectangular, thick, light transmission is better; coverslips are covered in the material, to avoid contact with the objective lens and to avoid contamination of the objective lens, was square , Thinner, better light transmission.
1, smear method is the material evenly coated on the slide on a production method.
Smear materials are single cell organisms, small algae, blood, bacterial culture medium, animal and plant loose tissue, testis, anthers and so on.
Smear should be noted: (1) slide must be clean. (2) The slides are flat. (3) the coating should be uniform. Smear the droplets in the middle of the slide, with a knife or toothpick, (4) the coating is thinner. Use another slide to push the film, along the drop of the coating of the glass slide (two slide angle should be 30 ° -45 °) from right to left gently push, coated evenly thin layer. (5) fixed. For fixation can be fixed with chemical fixative or dry method (bacteria). (6) staining. Bacteria with methylene blue, blood with Wright's dye, and sometimes you can use iodine. Dye to cover all the coating. (7) rinse. Dry or dry with absorbent paper. (8) Seal. Long-term preservation with a Canadian gum seal.
2, compression method is placed in the biological material between the slide and the cover sheet, the application of a certain pressure, the tissue cells of a preparation method.
3, loading method is the biological material to take the whole package into a glass specimen method, this method can be made into temporary or permanent film.
Filling materials are: tiny creatures such as Chlamydomonas sp., Sponge, amoeba, nematode; hydra, plant leaf epidermis; insect wings, feet, mouthparts, human oral epithelial cells.
Quartz Glass Slides Please note that:
(1) hand-held slide, should pay attention to flat, or on the platform. Drip water should be appropriate to cover the cover was just enough for the degree. (2) The material should be spread with anatomical needles or tweezers without overlapping, flattened on the same plane. (3) put the slide, from the side slowly covered in the water droplets, to prevent the emergence of bubbles. (4) dyeing, a drop of stained droplets on the side of the coverslip, with absorbent paper from the other side of the draw, so that the specimen under the cover glass uniform coloring. After coloring, with the same method, drop a drop of water, the dye solution after the suction, observed under a microscope.
4, slices are slices made of slices cut from the organism.
Depending on the requirements, the blade can be sliced by hand, the tissue can also be embedded in paraffin or lap or frozen at low temperature, sliced with a slicer. Cut into thin slices of 5 to 10 microns for optical microscopy. Ultra-thin slices cut with epoxy or methacrylic acid-embedded tissue blocks with a thickness of 20 to 50 nm, specifically for observation under an electron microscope. General teachings such as root tips, stem slices commonly known as paraffin sections.